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With over a decade of experience in marker-assisted breeding projects, DNA LandMarks has had the opportunity to participate in some tremendous advances in our clients’ breeding programs.
Case Study: Improving Yield in Popping Corn
- Morphology of popcorn limits yield
- Project focused on identifying and mapping "popping" QTL
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QTL analysis
- Cross yellow dent variety with a popping variety
- 3 F2 populations (250 plants each)
- 15 traits assessed in F3 families segregating for popping QTL
- 12-13 QTL identified for various popping traits
- Traits tracked using linked SSR markers
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Once popping QTL had been identified and linked to SSRs, two populations were created using a popping donor parent and a yellow dent recurrent parent.
- BC1: Two populations
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- Sample size: 13,442 (population 1) & 10,152 (population 2)
- Stepwise selection with 12-13 QTL-linked markers for heterozygosity
- Background selection with SSR markers for Recurrent Parent
- BC2: Two populations
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- Sample size: 15,372 & 5,339
- Stepwise selection with 12-13 QTL-linked markers for heterozygosity
- Background selection with SSR markers for Recurrent Parent
- BC2S1:
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- Sample size: 1,139 & 976
- Selection with 12-13 QTL-linked markers to fix the donor alleles
- BC2S2:
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- Sample size: 563 & 1,038
- Further selection with markers to fix the donor alleles
- IMP genotyping for Recurrent Parent - 87% similarity
BC2S2 x Testers: Field trials
- Results:
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